PIRKLE-TYPE COLUMNS
Exclusive to Regis, we offer additional chiral phases with Pirkle-Type (or commonly
referred to as brush-type) chemistries. Pirkle chiral stationary phases generally
fall into three classes: π-electron acceptor/π-electron donors, the π-electron
acceptors and the π-electron donors. With Pirkle phases, chiral recognition
occurs at binding sites. Major binding sites are classified as π -basic or π -acidic
aromatic rings, acidic sites, basic sites, and steric interaction sites. Aromatic
rings are potential sites for π - π interactions. Acidic sites supply hydrogens
for potential intermolecular hydrogen bonds; the hydrogen is often an amido
proton (N-H) from an amide, carbamate, urea, or amine. Basic sites, such as
π-electrons, sulfinyl or phosphinyl oxygens, and hydroxy or ether oxygens, may
also be involved in hydrogen bond formation. Steric interactions may also occur
between large groups.
Advantages of the Pirkle-Type Chiral Stationary Phases
• Long lasting, robust columns
• Covalently bonded selector
• Compatible with strong solvents for cleaning
• Compatible with SFC and SMB applications
• High Capacity
*Alpha Burke 2*
This π-acceptor chiral stationary phase is particularly valuable in the HPLC separation of β-blocker enantiomers, an important class of cardiovascular drugs whose enantiomers often exhibit differing pharmacological activities. The α-Burke 2 has been specifically designed to separate the enantiomers of β-blockers without chemical derivatization. In addition, it also resolves the enantiomers of many compounds separated on π-acceptor Pirkle type chiral stationary phases.